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Nov, 2022. Students have to make their own Unique BIO204 Assignment 1 Solution
Fall 2022. BIO204 - Principles of Biochemical Engineering Assignment 1 based on
one question & total marks are 10.
Question No. 1:
Describe the microbial growth kinetics of Batch
Culture?
Answer:
Growth → Change in
state/number/size/volume or in other sense it is the ratio between birth (B)
and death (D). Growth kinetics refers to the change in number or size of
organisms with respect to time.
In batch processing, a batch of culture medium in a
fermenter is inoculated with a microorganism (the ‘starter culture’). Fermentation
proceeds for a certain duration (the ‘fermentation time’ or ‘batch time’), and
the product is harvested at the end.
§ It is a closed
system which contains an initial limited amount of nutrient. §We put all the things
at one time here and ‘s’ (concentration of substrate) will remain same.
§Minor change in
volume.
OR
Batch growth kinetics of a microbe follows a growth
curve with lag phase as the initial phase during which cells adapt to a new
environment. Multiple lag phases occur if the media is supplemented with more
than one sugar and such type of growth is referred to as diauxic growth.
Μmax: maximum
specific growth rate (h-1)
Xo: initial biomass
concentration (mg/ml)
Xmax: maximum biomass
concentration (mg/ml)